Abstract Addressing the political subjectivations in the June 2013 Journeys, this study aims to analyze the political formation of young people based on their experience participating in 2013 demonstrations and its influences in the reconstruction of their political and educational identities and trajectories. In dialogue with the notions of itineraries and trajectories, a bibliographic review was carried out on results that dealt with the 2013 Journeys regarding the themes of youth, students, education, subjectivities, and identities. As theoretical framework, Jacques Rancière’s notion of political subjectivation guides our formulation of research questions, which are described as our main results. Thus, as results, rather than conclusions, this study build analysis scripts to guide field research on “Educational dimensions of the 2013 Journeys: Educational agendas, school experiences, and political formation of young people in protest,” which will interview militants and activists in 10 Brazilian states on questions about the tensions and approximations between the contingent experiences of protesting (which created moments of political subjectivation), coexistence in a “multitude” of multiple identities that are affirmed and rebuilt. Also, questions on the ambiguities and contradictions of the Journeys — expressed in the bifurcation of protest paths in the following years between continuing progressive demonstrations and the conservative drift that impeached Dilma in 2016 and elected a candidate of the extreme right in 2018.

Resumo Tendo como tema as subjetivações políticas nas Jornadas de Junho de 2013, o artigo objetiva analisar a formação política de jovens propiciada pela experiência de participação nas manifestações, bem como suas influências na reconstrução de identidades e trajetórias políticas e educacionais. Em diálogo com as noções de itinerários e trajetórias, é feita uma revisão bibliográfica sobre produtos que trataram das Jornadas de 2013 nos temas juventude, estudantes, educação, subjetivações e identidades. Como referencial teórico, a noção de subjetivação política de Jacques Rancière orienta a formulação de questões de pesquisa, as quais são apresentadas como principais resultados. Assim, como resultados, mais do que conclusões, o artigo constrói roteiros de análise que orientarão a pesquisa de campo para a pesquisa “Dimensões educacionais das Jornadas de 2013: Pautas educacionais, experiências escolares e formação política de jovens em protesto”, quando serão entrevistados militantes e ativistas em dez estados brasileiros. Destacam-se as interrogações sobre as tensões e aproximações entre, de um lado, as experiências contingentes de participação nos protestos – que criaram momentos de subjetivação política – e, de outro, a convivência, em “multidão”, de múltiplas identidades que são afirmadas e reconstruídas. Também, interrogações a respeito das ambiguidades e contradições das Jornadas, expressas na bifurcação dos caminhos dos protestos nos anos seguintes, entre a continuidade de manifestações progressistas e a deriva conservadora que implicou no impeachment de Dilma, em 2016, e na eleição do candidato da extrema-direita, em 2018.

Abstract The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed’s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.

RESUMO O objetivo deste estudo foi avaliar a dinâmica alimentar e fisiológica de novilhas Pantaneira e Girolando criadas em pastagem e a preferência por três tipos de sombras naturais. Foram utilizados 14 animais, sete de cada raça, com peso médio de 328 kg (Pantaneira) e 430 kg (Girolando). O pasto consistia em Panicum maximum. Foi avaliado o comportamento dos animais em áreas abertas e sob as copas das árvores (Anadenanthera colubrina; Terminalia argentea e Mangifera indica). Duas observações de 24 horas foram realizadas por estação, totalizando 192 horas de coleta de dados. O delineamento experimental foi inteiramente casualizado, utilizando arranjos fatorial 4 × 2 × 3 (estação × raça × copa) e 4 × 2 (estação e raça), com 7 repetições. M. indica foi a espécie arbórea preferida pelas duas raças em todas as estações. Os animais Girolando permaneceram mais tempo sob o dossel das árvores durante o inverno e a primavera. A dinâmica alimentar das duas raças foi semelhante, com efeito sazonal nas variáveis de ruminação em pé (RP) e interação social, com Girolando gastando mais tempo de RP na primavera (230,0 min) e menor interação no verão (53,57 min). Animais Girolando procuraram por água com mais frequência durante o verão. Não foram encontradas diferenças estatísticas ou interações entre fatores para micção e defecação dos animais, exceto para a defecação no período de verão, que foi maior que nas demais estações.

ABSTRACT The objective of this study was to evaluate feeding and physiological dynamics of Pantaneira and Girolando heifers reared on pasture and their preference for three types of natural shadows. Fourteen animals, seven of each breed, with mean weight of 328 kg (Pantaneira) and 430 kg (Girolando) were used. The pasture consisted of Panicum maximum; the animals' behavior in open areas and under treetops (Anadenanthera colubrina; Terminalia argentea, and Mangifera indica) was evaluated. Two 24-hour observations were performed per station, totaling 192 hours of data collection. The experimental design was completely randomized, using 4×2×3 (station × breed × canopy) and 4×2 (station and breed) factorial arrangements, with 7 replications. M. indica was the preferred tree species by both breeds in all seasons; Girolando animals remained longer under the canopy of these trees during winter and spring. Feeding dynamics of the two breeds was similar, with a seasonal effect on the standing rumination (SR) variables and social interaction, with Girolando spending more SR time in the spring (230.0 min) and less interaction in the summer (53.57min). Girolando animals searched for water more often during summer. No statistical differences or interactions between factors were found for urination and defecation of the animals, except for defecation in the summer period, which was higher than in the other seasons.

AbstractA device comprising a lab-made chamber with mechanical stirring and computer-controlled solenoid valves is proposed for the mechanization of liquid-liquid extractions. The performance was demonstrated by the extraction of ethanol from biodiesel as a model of the extraction of analytes from organic immiscible samples to an aqueous medium. The volumes of the sample and extractant were precisely defined by the flow-rates and switching times of the valves, while the mechanic stirring increased interaction between the phases. Stirring was stopped for phase separation, and a precise time-control also allowed a successful phase separation (i.e., the absence of the organic phase in the aqueous extract). In the model system, a linear response between the analytical response and the number of extractions was observed, indicating the potential for analyte preconcentration in the extract. The efficiency and reproducibility of the extractions were demonstrated by recoveries of ethanol spiked to biodiesel samples within 96% and 100% with coefficients of variation lower than 3.0%.

A sensitive spectrophotometric method was developed for sulphate determination in automotive ethanol fuel. The method based on the reaction of the analyte with barium-dibromosulphonazo(III) complex lead to a decrease in the magnitude of the absorbance signals monitored at 649 nm. No sample pretreatment is required and the proposed method allows sulphate determination in the 0.45 - 6.50 mg L-1 range with R.S.D. < 2% and limit of detection of 0.14 mg L-1. The method has been successfully applied for sulphate determination in automotive ethanol fuel and the results agreed with the reference chromatographic method.